Herein, we developed a lab-based, quick-processing and cost-effective fabrication method using lift-off process combined with anodic bonding method, which opts out of using any etching methods. However, almost all microfluidic models were fabricated by using etching methods and rare ones were having dual-scale micro-/nanofluidic channels. Microfluidic model, as a potential powerful tool, has been used for decades for investigating fluid flow at pore-scale in energy field. Unconventional shale or tight oil/gas reservoirs that have micro-/nano sizes of the dual-scale matrix pore throats with micro-fractures may result in different fluid flow mechanisms compared with conventional oil/gas reservoirs. Thus, this methodology could be applied to the rational design of lab-on-a-chip devices for any magnetically driven purification, enrichment or isolation. Our results indicate that rectangular, long devices display the best performance as they deliver high particle recovery and high throughput. For that purpose, we employ an experimentally validated Computational Fluid Dynamics (CFD) numerical model that considers the dominant forces acting on the beads during separation. The influence of several geometrical features (namely cross section shape, thickness, length, and volume) on both bead recovery and system throughput is studied. Herein, we address the optimization of Y-Y-shaped microchannels, where magnetic beads are separated from blood and collected into a buffer stream by applying an external magnetic field. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle capture however, less attention has been paid to the effect of the channel geometry on the system performance, although it is key for designing systems that simultaneously provide high particle recovery and flow rates. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. The study indicated that the fabricating microstructure of glass microfluidic chip could be finished in 12 min with good surface quality, thus, providing a promising method for achieving mass production of glass microfluidic chips in the future. Finally, in order to verify the performance of the molded chips by the GMP, a mixed microfluidic chip was chosen to conduct an actual liquid filling experiment. The analysis of mold wear was then conducted by the comparison of mold morphology, before and after the GMP, which indicated that the mold was suitable for GMP. Next, the molds for fabricating three typical microfluidic chips, for example, diffusion mixer chip, flow focusing chip, and cell counting chip, were prepared and used to mold microfluidic chips. Firstly, a mold with protrusion microstructure was prepared and used to fabricate grooves to evaluate the GMP performance in terms of roughness and height. This paper investigated the glass molding process (GMP) for fabricating microstructures of microfluidic chips. Compared with polymer-based biochips, such as polydimethylsiloxane (PDMS), glass based chips have drawn much attention due to their high transparency, chemical stability, and good biocompatibility.
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